Mass Spectrometry Facility

Mass Spectrometry Facility

 Facility Resources

Mass spectrometry resources housed within Wake Forest University’s Department of Chemistry are utilized independently by individual, trained users. Instrumentation includes a high resolution Thermo Orbitrap LTQ XL (ESI, APCI) with Accela UPLC, an Agilent ion trap (ESI, APCI) with HPLC, and two Agilent GC-MS (EI, CI). The facility also has a walk-up dedicated workstation for database searching, most often using Proteome Discoverer.

Use of MS instruments is freely available to trained WFU students and faculty members of the Department of Chemistry and their direct collaborators. All instruments are operated on a by-reservation basis (24/7) using the Central Reservation System in the Important Links tab.

For questions regarding accessibility if outside of WFU Chem:  Dr. Mark Welker, Department Chair.

 Instrumentation

Thermo Orbitrap LTQ XL with Accela UPLC and autosampler

Thermo Orbitrap LTQ XL with Accela UPLC and autosampler

Agilent 1100 LC/MSD with autosampler:

Agilent 1100 LC/MSD with autosampler:

Agilent 7890 GC/5975 MS with autosampler:

Agilent 7890 GC/5975 MS with autosampler:

Mass spectrometry workstation (Proteome Discoverer)

Mass spectrometry workstation (Proteome Discoverer)

 Important Links

MNova MS Training Handout

WFU-developed MNova tutorial videos (coming soon!)

Access to Central Instrument Reservation System

 MS User Resources

High Res Mass Calculator (http://www.alchemistmatt.com/mwtwin.html)

ProteinProspector – predict fragmentation, isotope patterns, modifications (http://prospector.ucsf.edu/prospector/mshome.htm)

ExPASy – proteomics links and free specialized software (http://www.expasy.org/proteomics)

FASTA Files (http://www.uniprot.org/taxonomy/complete-proteomes)

Thermo Brims Portal (http://portal.thermo-brims.com/)

Troubleshooting chromatography (http://www.proteinsandproteomics.org/content/free/protocols_1/pro04-d.html)

Proteomics blog with training videos, tutorials, tips & tricks (http://proteomicsnews.blogspot.com/)

 MestReNova Tutorial


“Created by Jason Pifer, PhD candidate, March 2015”